Determination of Alanine Aminotransferase with an Electrochemical Nano Ir-C Biosensor for the Screening of Liver Diseases

Biosensors

Chang-Jung Hsueh¹  Joanne H. Wang²  Liming Dai¹ 

[1] Department of Chemical Engineering and Electronics Design Center, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 44106, USA; E-Mails:;Department of Biology, Brown University, 69 Brown Street, Providence, RI 02912, USA; E-Mail:

关键词: liver diseases;    alanine aminotransferase;    electrochemical detection;    biosensor;   

DOI  :  10.3390/bios1030107

来源: mdpi

【 摘 要 】

Alanine aminotransaminase (ALT), is an enzyme that normally resides in serum and body tissues, especially in the liver. It is released into the serum as a result of tissue injury; hence the concentration of ALT in the serum may be increased with acute damage to hepatic cells. A single use, disposable biosensor, comprising iridium nano-particle as catalyst dispersed on carbon paste, has been developed for the determination of ALT concentration. The biosensor is based on quantifying H₂O₂ concentration produced by a serial of ALT enzymatic reactions. It operates well at room temperature in different physiological fluids: phosphate buffer, calf serum and human serum for ALT concentration of 0–544 ng/mL. Experimental results in human serum are compared to those obtained by spectrophotometric assays with excellent agreement. Therefore, the Ir/C biosensor shows good relationship on the dilution of concentrated ALT clinical applications.

【 授权许可】

CC BY   
© 2011 by the authors; licensee MDPI, Basel, Switzerland.