Development of an Electrochemical-Based Aspartate Aminotransferase Nanoparticle Ir-C Biosensor for Screening of Liver Diseases

Biosensors

Chang-Jung (Alan) Hsueh¹  Joanne H. Wang²  Liming Dai³ 

[1] Department of Chemical Engineering and Electronics Design Center, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 44106, USA; E-Mail:;Warren Alpert Medical School of Brown University, 222 Richmond Street, Providence, RI 02912, USA; E-Mail:;Department of Macromolecular Science and Engineering, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 44106, USA; E-Mail:

关键词: liver diseases;    aspartate aminotransferase;    electrochemical detection;    amperometric biosensor;   

DOI  :  10.3390/bios2020234

来源: mdpi

【 摘 要 】

Aspartate aminotransaminase (AST) is a hepatocelluar enzyme released into the bloodstream when hepatic cells are damaged, resulting in elevated blood levels of AST. A single use, disposable biosensor prototype, composed of catalytic iridium nano-particles dispersed on carbon paste, was developed to detect enzymatically-produced H₂O₂ in AST-mediated reactions. This biosensor is capable of measuring AST levels in a phosphate buffer and undiluted human serum over the concentration range of 0 to 0.89 μg/mL AST concentration (corresponding to 0–250 UL⁻¹ specific activity). The biosensor operates at relatively low oxidation potential (+0.3 volt (V) versus the printed Ag/AgCl), minimizing any potential chemical interference in human serum. The measurements of AST in human serum using the biosensor compared well with those measured by standard hospital spectrophotometric assays. This Ir-C biosensor may be useful for AST measurements in the clinical environment.

【 授权许可】

CC BY   
© 2012 by the authors; licensee MDPI, Basel, Switzerland.