The Detection of Alkaline Phosphatase Using an Electrochemical Biosensor in a Single-Step Approach

Sensors

Joanne H. Wang³  Kevin Wang²  Brandon Bartling¹ 

[1] Department of Chemical Engineering, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 44106, USA; E-Mail:;College of Medicine, University of Cincinnati, 231 Albert B. Sabin Way, Cincinnati, OH 45267, USA; E-Mail:;Department of Biology, Brown University, 171 Meeting Street, Providence, RI 02912, USA; E-Mail:

关键词: alkaline phosphatase;    electrochemical detection;    biosensor;   

DOI  :  10.3390/s91108709

来源: mdpi

【 摘 要 】

A one-step, single use, disposable Alkaline Phosphatase (ALP) biosensor has been developed. It is based on the detection of phenol produced by an ALP enzymatic reaction. It can operate at 25 °C in a pH 10 medium. It measures ALP of 0–300 IU/L. The permissible concentrations of glucose, ascorbic acid and urea without interference are 10 mM/L, 5 mg/L and 400 mg/L, respectively. Experimental results are compared to those obtained by spectrophotometric measurements in bovine serum. Excellent linearity between the biosensor outputs and the ALP concentrations exists. The agreement between the measurements of this biosensor and the spectrophotometer is also outstanding.

【 授权许可】

CC BY   
© 2009 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.