Enhancing Single Molecule Imaging in Optofluidics and Microfluidics

International Journal of Molecular Sciences

Andreas E. Vasdekis¹ 

关键词: optofluidics;    microfluidics;    single molecule;    fluorescence;    imaging;    surface passivation;    micro-fabrication;    lab-on-a-chip;   

DOI  :  10.3390/ijms12085135

来源: mdpi

【 摘 要 】

Microfluidics and optofluidics have revolutionized high-throughput analysis and chemical synthesis over the past decade. Single molecule imaging has witnessed similar growth, due to its capacity to reveal heterogeneities at high spatial and temporal resolutions. However, both resolution types are dependent on the signal to noise ratio (SNR) of the image. In this paper, we review how the SNR can be enhanced in optofluidics and microfluidics. Starting with optofluidics, we outline integrated photonic structures that increase the signal emitted by single chromophores and minimize the excitation volume. Turning then to microfluidics, we review the compatible functionalization strategies that reduce noise stemming from non-specific interactions and architectures that minimize bleaching and blinking.

【 授权许可】

CC BY   
© 2011 by the authors; licensee MDPI, Basel, Switzerland.