Isolation and Purification of Oridonin from the Whole Plant of Isodon rubescens by High-Speed Counter-Current Chromatography

Molecules

Fa He¹  Yuhua Bai¹  Jing Wang¹  Jing Wei¹  ChunYue Yu¹  Sen Li¹  Weili Yang¹ 

[1] ¹College of Pharmacy, Harbin University, Daqing 163319, China ²Petrochemical Research Institute Daqing Petrochemical Research Center, Daqing 163714, China

关键词: Isodon rubescens;    high-speed counter-current chromatography (HSCCC);    oridonin;   

DOI  :  10.3390/molecules16097949

来源: mdpi

【 摘 要 】

Semi-preparative high-speed counter-current chromatography (HSCCC) was successfully used for isolation and purification of oridonin from Isodon rubescens by using a two-phase-solvent system composed of n-hexane-ethyl acetate-methanol-water (2.8:5:2.8:5, v/v/v/v). The targeted compound isolated, collected and purified by HSCCC was analyzed by high performance liquid chromatography (HPLC). A total of 40.6 mg of oridonin with the purity of 73.5% was obtained in less than 100 min from 100 mg of crude Isodon rubescens extract. The chemical structure of the compound was identified by IR, ¹H-NMR and ¹³C-NMR.

【 授权许可】

CC BY   
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.