Preparative Isolation of Three Anthraquinones from Rumex japonicus by High-Speed Counter-Current Chromatography

Molecules

Shuying Guo¹  Bo Feng¹  Ruonan Zhu¹  Jiankang Ma¹ 

[1] ¹College of Pharmacy, Jilin Medical College, Jilin 132013, China ²Institute of Phytochemistry, Jilin Academy of Chinese Medicine Sciences, Changchun 130012, China

关键词: Rumex japonicus Houtt.;    anthraquinones;    high-speed counter-current chromatography (HSCCC);    emodin;    chrysophanol;    physcion;   

DOI  :  10.3390/molecules16021201

来源: mdpi

【 摘 要 】

Three anthraquinones—emodin, chrysophanol, and physcion—were successfully purified from the dichloromethane extract of the Chinese medicinal herb Rumex japonicus by high-speed counter-current chromatography (HSCCC). The extract was separated with n-hexane–ethanol–water (18:22:3, v/v/v) as the two-phase solvent system and yielded 3.4 mg of emodin, 24.1 mg of chrysophanol, and 2.0 mg of physcion from 500 mg of sample with purities of 99.2 %, 98.8% and 98.2%, respectively. The HSCCC fractions were analyzed by high-performance liquid chromatography (HPLC) and the chemical structures of the three anthraquinones were confirmed by ¹H-NMR and ¹³C-NMR analysis. This is the first time these anthraquinones have been obtained from R. japonicus by HSCCC.

【 授权许可】

CC BY   
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.