Bioactive Compounds and Biological Activities of Jatropha curcas L. Kernel Meal Extract

International Journal of Molecular Sciences

Ehsan Oskoueian²  Norhani Abdullah³  Syahida Ahmad³  Wan Zuhainis Saad²  Abdul Rahman Omar¹ 

[1] Institute of Bioscience, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia; E-Mails:;Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia; E-Mails:;Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia; E-Mail:

关键词: Jatropha curcas L.;    bioactive compounds;    antibacterial;    antioxidant;    cytotoxicity;    kernel meal;   

DOI  :  10.3390/ijms12095955

来源: mdpi

【 摘 要 】

Defatted Jatropha curcas L. (J. curcas) seed kernels contained a high percentage of crude protein (61.8%) and relatively little acid detergent fiber (4.8%) and neutral detergent fiber (9.7%). Spectrophotometric analysis of the methanolic extract showed the presence of phenolics, flavonoids and saponins with values of 3.9, 0.4 and 19.0 mg/g DM, respectively. High performance liquid chromatography (HPLC) analyses showed the presence of gallic acid and pyrogallol (phenolics), rutin and myricetin (flavonoids) and daidzein (isoflavonoid). The amount of phorbol esters in the methanolic extract estimated by HPLC was 3.0 ± 0.1 mg/g DM. Other metabolites detected by GC-MS include: 2-(hydroxymethyl)-2 nitro-1,3-propanediol, β-sitosterol, 2-furancarboxaldehyde, 5-(hydroxymethy) and acetic acid in the methanolic extract; 2-furancarboxaldehyde, 5-(hydroxymethy), acetic acid and furfural (2-furancarboxaldehyde) in the hot water extract. Methanolic and hot water extracts of kernel meal showed antimicrobial activity against both Gram positive and Gram negative pathogenic bacteria (inhibition range: 0–1.63 cm) at the concentrations of 1 and 1.5 mg/disc. Methanolic extract exhibited antioxidant activities that are higher than hot water extract and comparable to β-carotene. The extracts tended to scavenge the free radicals in the reduction of ferric ion (Fe³⁺) to ferrous ion (Fe²⁺). Cytotoxicity assay results indicated the potential of methanolic extract as a source of anticancer therapeutic agents toward breast cancer cells.

【 授权许可】

CC BY   
© 2011 by the authors; licensee MDPI, Basel, Switzerland.