A Determination of Potential α-Glucosidase Inhibitors from Azuki Beans (Vigna angularis)

International Journal of Molecular Sciences

Yang Yao¹  Xuzhen Cheng¹  Lixia Wang¹  Suhua Wang¹ 

[1] Institute of Crop Science, Chinese Academy of Agricultural Sciences, South Xueyuan Road, Haidian District No.80, Beijing 100081, China;

关键词: azuki beans;    α-glucosidase inhibitory;    vitexin;    isovitexin;   

DOI  :  10.3390/ijms12106445

来源: mdpi

【 摘 要 】

A 70% ethanol extract from azuki beans (Vigna angularis) was extracted further with CH₂Cl₂, EtOAc and n-BuOH to afford four fractions: CH₂Cl₂-soluble, EtOAc-soluble, n-BuOH-soluble and residual extract fractions. The EtOAc-soluble fractions showed the highest α-glucosidase inhibitory activity. Two pure flavonoid compounds, vitexin and isovitexin, were isolated (using the enzyme assay-guide fractionation method) from the EtOAc-soluble fractions. We further evaluated the interaction between the flavonoid compounds and α-glucosidase by fluorescence spectroscopy. Vitexin and isovitexin showed high inhibitory activities, with IC₅₀ values of 0.4 mg·mL⁻¹ and 4.8 mg·mL⁻¹, respectively. This is the first study of the active compositions of azuki beans against α-glucosidase.

【 授权许可】

CC BY   
© 2011 by the authors; licensee MDPI, Basel, Switzerland.