Following Glucose Oxidase Activity by Chemiluminescence and Chemiluminescence Resonance Energy Transfer (CRET) Processes Involving Enzyme-DNAzyme Conjugates

Sensors

Angelica Niazov¹  Ronit Freeman¹  Julia Girsh¹ 

[1] id="af1-sensors-11-10388">Institute of Chemistry, Center for Nanoscience and Nanotechnology, The Hebrew University of Jerusalem, Jerusalem 91904, Isra

关键词: sensor;    glucose;    chemiluminescence;    DNAzyme;    quantum dots;    hydrogen peroxide;    G-quadruplex;    chemiluminescence resonance energy transfer;   

DOI  :  10.3390/s111110388

来源: mdpi

【 摘 要 】

A hybrid consisting of glucose oxidase-functionalized with hemin/G-quadruplex units is used for the chemiluminescence detection of glucose. The glucose oxidase-mediated oxidation of glucose yields gluconic acid and H₂O₂. The latter in the presence of luminol acts as substrate for the hemin/G-quadruplex-catalyzed generation of chemiluminescence. The glucose oxidase/hemin G-quadruplex hybrid was immobilized on CdSe/ZnS quantum dots (QDs). The light generated by the hybrid, in the presence of glucose, activated a chemiluminescence resonance energy transfer process to the QDs, resulting in the luminescence of the QDs. The intensities of the luminescence of the QDs at different concentrations of glucose provided an optical means to detect glucose.

【 授权许可】

CC BY   
© 2011 by the authors; licensee MDPI, Basel, Switzerland.