A Sensitive DNAzyme-Based Chiral Sensor for Lead Detection

Materials

Hua Kuang¹  Honghong Yin²  Changrui Xing² 

[1] State Key Lab of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China;

关键词: lead;    detection;    DNAzyme;    circular dichroism (CD);    sensor;   

DOI  :  10.3390/ma6115038

来源: mdpi

【 摘 要 】

A DNAzyme-based sensor for the determination and quantification of lead ions (Pb²⁺) has been established, which combines the recognition and catalysis of DNAzyme with the optical properties of nanomaterials. Circular dichroism (CD) signals were obtained by a DNAzyme-based assembly of asymmetric silver nanoparticle (AgNPs) dimers. A good linear relationship between CD signals and Pb²⁺ concentration was obtained ranging from 0.05 ng∙mL⁻¹ to 10 ng∙mL⁻¹ with a limit of detection (LOD) of 0.02 ng∙mL⁻¹. The specificity of this sensor in lead ion detection was excellent, and a satisfactory recovery was obtained in the analysis of tap water samples. The proposed technique possesses both high sensitivity and good specificity, giving it great potential for the analysis of Pb²⁺ in water.

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland.