Sensors | |
Time-Resolved Fluorescence Immunoassay for C-Reactive Protein Using Colloidal Semiconducting Nanoparticles | |
Harri Härmä1  Juha Toivonen1  Juhani T. Soini1  Pekka Hänninen1  | |
[1] Laboratory of Biophysics, University of Turku, 20520 Turku, Finland; E-Mails: | |
关键词: colloidal nanoparticles; quantum dots; time-gated fluorescence; sensing; bioaffinity assays; | |
DOI : 10.3390/s111211335 | |
来源: mdpi | |
【 摘 要 】
Besides the typical short-lived fluorescence with decay times in the nanosecond range, colloidal II/VI semiconductor nanoparticles dispersed in buffer also possess a long-lived fluorescence component with decay times in the microsecond range. Here, the signal intensity of the long-lived luminescence at microsecond range is shown to increase 1,000-fold for CdTe nanoparticles in PBS buffer. This long-lived fluorescence can be conveniently employed for time-gated fluorescence detection, which allows for improved signal-to-noise ratio and thus the use of low concentrations of nanoparticles. The detection principle is demonstrated with a time-resolved fluorescence immunoassay for the detection of C-reactive protein (CRP) using CdSe-ZnS nanoparticles and green light excitation.
【 授权许可】
CC BY
© 2011 by the authors; licensee MDPI, Basel, Switzerland.
【 预 览 】
Files | Size | Format | View |
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RO202003190047003ZK.pdf | 207KB | download |