Time-Resolved Fluorescence Immunoassay for C-Reactive Protein Using Colloidal Semiconducting Nanoparticles

Sensors

Harri Härmä¹  Juha Toivonen¹  Juhani T. Soini¹  Pekka Hänninen¹ 

[1] Laboratory of Biophysics, University of Turku, 20520 Turku, Finland; E-Mails:

关键词: colloidal nanoparticles;    quantum dots;    time-gated fluorescence;    sensing;    bioaffinity assays;   

DOI  :  10.3390/s111211335

来源: mdpi

【 摘 要 】

Besides the typical short-lived fluorescence with decay times in the nanosecond range, colloidal II/VI semiconductor nanoparticles dispersed in buffer also possess a long-lived fluorescence component with decay times in the microsecond range. Here, the signal intensity of the long-lived luminescence at microsecond range is shown to increase 1,000-fold for CdTe nanoparticles in PBS buffer. This long-lived fluorescence can be conveniently employed for time-gated fluorescence detection, which allows for improved signal-to-noise ratio and thus the use of low concentrations of nanoparticles. The detection principle is demonstrated with a time-resolved fluorescence immunoassay for the detection of C-reactive protein (CRP) using CdSe-ZnS nanoparticles and green light excitation.

【 授权许可】

CC BY   
© 2011 by the authors; licensee MDPI, Basel, Switzerland.