A Highly Sensitive Time-Gated Fluorescence Immunoassay Platform Using Mn-Doped AgZnInS/ZnS Nanocrystals as Signal Transducers

Frontiers in Physics

David AuCoin¹  Derrick Hau¹  Brandon Gallian²  Xiaoshan Zhu²  Masoumeh Saber Zaeimian² 

[1] Reno, NV, United States;Reno, NV, United States;Reno, NV, United States;

关键词: nanocrystals;    Mn doping;    time-gated fluorescence;    immunoassay;    analytical platform;   

DOI  :  10.3389/fphy.2020.625424

来源: Frontiers

【 摘 要 】

In this work, a time-gated immunoassay platform using low-energy excitable and fluorescence long-lived Mn:AgZnInS/ZnS nanocrystals as signal transducers was developed and applied to the detection of the capsular polysaccharide (CPS) of Burkholderia pseudomallei, a Gram-negative bacterium that is the causative agent of melioidosis. CPS is a high molecular weight antigen displayed and is shed from the outer membrane of B. pseudomallei. The immunoassay using the time-gated platform presents a limit of detection at around 23 pg/ml when CPS is spiked in human serum.

【 授权许可】

CC BY