期刊论文详细信息
Frontiers in Physics | |
A Highly Sensitive Time-Gated Fluorescence Immunoassay Platform Using Mn-Doped AgZnInS/ZnS Nanocrystals as Signal Transducers | |
David AuCoin1  Derrick Hau1  Brandon Gallian2  Xiaoshan Zhu2  Masoumeh Saber Zaeimian2  | |
[1] Reno, NV, United States;Reno, NV, United States;Reno, NV, United States; | |
关键词: nanocrystals; Mn doping; time-gated fluorescence; immunoassay; analytical platform; | |
DOI : 10.3389/fphy.2020.625424 | |
来源: Frontiers | |
【 摘 要 】
In this work, a time-gated immunoassay platform using low-energy excitable and fluorescence long-lived Mn:AgZnInS/ZnS nanocrystals as signal transducers was developed and applied to the detection of the capsular polysaccharide (CPS) of Burkholderia pseudomallei, a Gram-negative bacterium that is the causative agent of melioidosis. CPS is a high molecular weight antigen displayed and is shed from the outer membrane of B. pseudomallei. The immunoassay using the time-gated platform presents a limit of detection at around 23 pg/ml when CPS is spiked in human serum.
【 授权许可】
CC BY
【 预 览 】
Files | Size | Format | View |
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RO202107215991006ZK.pdf | 1303KB | download |