An Investigation into the Cytotoxic Effects of 13-Acetoxysarcocrassolide from the Soft Coral Sarcophyton crassocaule on Bladder Cancer Cells

Marine Drugs

Ching-Chyuan Su⁴  Jui-Hsin Su²  Jen-Jie Lin³  Cheng-Chi Chen³  Wen-Ing Hwang¹  Han Hsiang Huang³ 

[1] Department of Food Science and Nutrition, Meiho University, Pingtung 91202, Taiwan;National Museum of Marine Biology and Aquarium, Pingtung 94446, Taiwan;Department of Beauty Science, Meiho University, Pingtung 91202, Taiwan;Antai Medical Care Cooperation Antai Tian-Sheng Memorial Hospital, Pingtung 92842, Taiwan;

关键词: 13-acetoxysarcocrassolide;    Sarcophyton crassocaule;    BFTC cells;    proteomic analysis;   

DOI  :  10.3390/md9122622

来源: mdpi

【 摘 要 】

Active compounds from natural products have been widely studied. The anti-tumor effects of 13-acetoxysarcocrassolide isolated from Formosan soft coral Sarcophyton crassocaule on bladder cancer cells were examined in this study. An MTT assay showed that 13-acetoxysarcocrassolide was cytotoxic to bladder female transitional cancer (BFTC) cells. We determined that the BFTC cells underwent cell death through apoptosis by flow cytometry. Due to the highly-migratory nature of the BFTC cells, the ability of 13-acetoxysarcocrassolide to stop their migration was assessed by a wound healing assay. To determine which proteins were affected in the BFTC cells upon treatment, a comparative proteomic analysis was performed. By LC-MS/MS analysis, we identified that 19 proteins were up-regulated and eight were down-regulated. Seven of the proteins were confirmed by western blotting analysis. This study reveals clues to the potential mechanism of the cytotoxic effects of 13-acetoxysarcocrassolide on BFTC cells. Moreover, it suggests that PPT1 and hnRNP F could be new biomarkers for bladder cancer. The results of this study are also helpful for the diagnosis, progression monitoring and therapeutic strategies of transitional cell tumors.

【 授权许可】

CC BY   
© 2011 by the authors; licensee MDPI, Basel, Switzerland.