N-Acyl Homoserine Lactone Production by Klebsiella pneumoniae Isolated from Human Tongue Surface

Sensors

Wai-Fong Yin¹  Kathiravan Purmal³  Shenyang Chin¹  Xin-Yue Chan¹  Chong-Lek Koh²  Choon-Kook Sam² 

[1] Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia; E-Mails:;Natural Sciences and Science Education AG, National Institute of Education, Nanyang Technological University, 1 Nanyang Walk, 637616, Singapore; E-Mails:;School of Dental Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia; E-Mail:

关键词: N-acylhomoserine lactone;    N-3-dodecanoyl-l-homoserine lactone (C12-HSL);    N-heptanoyl-l-homoserine lactone (C7-HSL);    N-octanoyl-l-homoserine lactone (C8-HSL);    oral cavity;    posterior dorsal surface;    quorum sensing;    tongue;   

DOI  :  10.3390/s120303472

来源: mdpi

【 摘 要 】

Bacteria communicate by producing quorum sensing molecules called autoinducers, which include autoinducer-1, an N-hexanoyl homoserine lactone (AHL), and autoinducer-2. Bacteria present in the human oral cavity have been shown to produce autoinducer-2, but not AHL. Here, we report the isolation of two AHL-producing Klebsiella pneumoniae strains from the posterior dorsal surface of the tongue of a healthy individual. Spent culture supernatant extracts from K. pneumoniae activated the biosensors Agrobacterium tumefaciens NTL4(pZLR4) and Escherichia coli [pSB401], suggesting the presence of both long and short chain AHLs. High resolution mass spectrometry analyses of these extracts confirmed that both K. pneumoniae isolates produced N-octanoylhomoserine lactone and N-3-dodecanoyl-l-homoserine lactone. To the best of our knowledge, this is the first report of the isolation of K. pneumoniae from the posterior dorsal surface of the human tongue and the production of these AHLs by this bacterium.

【 授权许可】

CC BY   
© 2012 by the authors; licensee MDPI, Basel, Switzerland