Quorum Sensing Activity of Hafnia alvei Isolated from Packed Food

Sensors

Jia-Yi Tan¹  Wai-Fong Yin¹ 

[1] Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur 50603, Malaysia;

关键词: Hafnia alvei;    mass spectrometry;    N-acylhomoserine lactone;    N-(3-oxohexanoyl) homoserine lactone;    N-(3-oxooctanoyl) homoserine lactone;    quorum sensing;    fish products;    food spoilage;    food microbiology;    food safety;   

DOI  :  10.3390/s140406788

来源: mdpi

【 摘 要 】

Quorum sensing (QS) is a mechanism adopted by bacteria to regulate expression of genes according to population density. N-acylhomoserine lactones (AHLs) are a type of QS signalling molecules commonly found in Gram-negative bacteria which have been reported to play a role in microbial spoilage of foods and pathogenesis. In this study, we isolated an AHL-producing Hafnia alvei strain (FB1) from spherical fish pastes. Analysis via high resolution triple quadrupole liquid chromatography/mass spectrometry (LC/MS) on extracts from the spent supernatant of H. alvei FB1 revealed the existence of two short chain AHLs: N-(3-oxohexanoyl) homoserine lactone (3-oxo-C6-HSL) and N-(3-oxo- octanoyl) homoserine lactone (3-oxo-C8-HSL). To our knowledge, this is the first report of the production of AHLs, especially 3-oxo-C8-HSL, by H. alvei.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.