Very High Throughput Electrical Cell Lysis and Extraction of Intracellular Compounds Using 3D Carbon Electrodes in Lab-on-a-Chip Devices

Micromachines

Guillaume Mernier¹  Rodrigo Martinez-Duarte¹  Rajwinder Lehal²  Freddy Radtke² 

[1] Microsystems Laboratory LMIS4, Ecole Polytechnique Fédérale de Lausanne (EPFL), Station 17, CH-1015 Lausanne, Switzerland;Swiss Institute for Experimental Cancer Research, Ecole Polytechnique Fédérale de Lausanne (EPFL), Station 19, Lausanne, Vaud 1015, Switzerland;

关键词: microfluidics;    cell lysis;    carbon electrodes;   

DOI  :  10.3390/mi3030574

来源: mdpi

【 摘 要 】

Here we present an electrical lysis throughput of 600 microliters per minute at high cell density (10⁸ yeast cells per ml) with 90% efficiency, thus improving the current common throughput of one microliter per minute. We also demonstrate the extraction of intracellular luciferase from mammalian cells with efficiency comparable to off-chip bulk chemical lysis. The goal of this work is to develop a sample preparation module that can act as a stand-alone device or be integrated to other functions already demonstrated in miniaturized devices, including sorting and analysis, towards a true lab-on-a-chip.

【 授权许可】

CC BY   
© 2012 by the authors; licensee MDPI, Basel, Switzerland.