A Review on Macroscale and Microscale Cell Lysis Methods

Micromachines

Aditya Aryasomayajula¹  Mohammed Shehadul Islam¹  Ponnambalam Ravi Selvaganapathy¹ 

[1] Department of Mechanical Engineering, McMaster University, Hamilton, ON L8S 4L7, Canada;

关键词: cell lysis;    cell lysis methods;    microfluidics;    electrical lysis;    mechanical lysis;    thermal lysis;   

DOI  :  10.3390/mi8030083

来源: DOAJ

【 摘 要 】

The lysis of cells in order to extract the nucleic acids or proteins inside it is a crucial unit operation in biomolecular analysis. This paper presents a critical evaluation of the various methods that are available both in the macro and micro scale for cell lysis. Various types of cells, the structure of their membranes are discussed initially. Then, various methods that are currently used to lyse cells in the macroscale are discussed and compared. Subsequently, popular methods for micro scale cell lysis and different microfluidic devices used are detailed with their advantages and disadvantages. Finally, a comparison of different techniques used in microfluidics platform has been presented which will be helpful to select method for a particular application.

【 授权许可】

Unknown