Preparation of Dry Extract of Mikania glomerata Sprengel (Guaco) and Determination of Its Coumarin Levels by Spectrophotometry and HPLC-UV

Molecules

Luciana Soares e Silva¹  Luciane Santos da Silva¹  Larissa Brumano¹  Paulo César Stringheta³  Miriam Aparecida de Oliveira Pinto¹  Leticia Oliveira Moreira Dias¹  Camila de Sá Martins Muller¹  Elita Scio²  Rodrigo Luiz Fabri²  Helena C. Castro⁴ 

[1] Faculty of Pharmacy, Federal University of Juiz de Fora, Juiz de Fora 36036-900, MG, Brazil;Institute of Biological Sciences, Federal University of Juiz de Fora, Juiz de Fora 36036-900, MG Brazil;Federal University of Viçosa, Viçosa 36570-900, MG, Brazil;Institute of Biology, Fluminense Federal University, Niterói 24220-900, RJ, Brazil;

关键词: pharmacotechnique;    freeze-dryer;    lyophilization;    coumarins;   

DOI  :  10.3390/molecules170910344

来源: mdpi

【 摘 要 】

Guaco (Mikania glomerata Sprengel) syrup is one of the most popular herbal medicines used to treat the symptoms of asthmatic bronchitis, cough and hoarseness. The coumarin 2H-1-benzopyran-2-one, is one of the major constituents of Guaco and contributes to its pharmacological effects. The pharmaceutical capsule form of dry extract of Guaco is recommended by the Brazilian Program of Medicinal Plants and Herbal Medicines and used in primary health care. In order to identify a new protocol to obtain the raw material for Guaco capsule production we evaluated two methods, including a freeze-drying process (lyophilization) and the spray-dryer technique, as well as the use of two adjuvants, Maltodextrins and Aerosil^®, in different concentrations. The coumarin levels of the dried extracts were analyzed by UV-spectrophotometry and HPLC-UV/DAD. The adjuvant Aerosil^® 8% showed better dry powder physical appearance. Lyophilization was observed to be the best process to obtain the dry extract of Guaco based on the measured coumarin levels.

【 授权许可】

CC BY   
© 2012 by the authors; licensee MDPI, Basel, Switzerland.