期刊论文详细信息
International Journal of Molecular Sciences
Lyophilized Platelet-Rich Fibrin (PRF) Promotes Craniofacial Bone Regeneration through Runx2
Qi Li1  David A. Reed3  Liu Min2  Gokul Gopinathan3  Steve Li3  Smit J. Dangaria4  Leo Li3  Yajun Geng1  Maria-Therese Galang5  Praveen Gajendrareddy6  Yanmin Zhou1  Xianghong Luan3 
[1] Department of Implantology, Stomatological Hospital, Jilin University, Changchun 130021, Jilin, China; E-Mails:;Department of Periodontology, Stomatological Hospital, Jilin University, Changchun 130021, Jilin, China; E-Mail:;UIC Brodie Laboratory for Craniofacial Genetics, Chicago, IL 60612, USA; E-Mails:;College of Dentistry, University of Southern California, Los Angeles, CA 90089, USA; E-Mail:;Department of Orthodontics, College of Dentistry, University of Illinois at Chicago, Chicago, IL 60612, USA; E-Mail:;Department of Periodontics, College of Dentistry, University of Illinois at Chicago, Chicago, IL 60612, USA; E-Mail:
关键词: Platelet-rich fibrin;    lyophilization;    calvaria;    alveolar bone;    Runx2;   
DOI  :  10.3390/ijms15058509
来源: mdpi
PDF
【 摘 要 】

Freeze-drying is an effective means to control scaffold pore size and preserve its composition. The purpose of the present study was to determine the applicability of lyophilized Platelet-rich fibrin (LPRF) as a scaffold for craniofacial tissue regeneration and to compare its biological effects with commonly used fresh Platelet-rich fibrin (PRF). LPRF caused a 4.8-fold ± 0.4-fold elevation in Runt-related transcription factor 2 (Runx2) expression in alveolar bone cells, compared to a 3.6-fold ± 0.2-fold increase when using fresh PRF, and a more than 10-fold rise of alkaline phosphatase levels and mineralization markers. LPRF-induced Runx2 expression only occurred in alveolar bone and not in periodontal or dental follicle cells. LPRF also caused a 1.6-fold increase in osteoblast proliferation (p < 0.001) when compared to fresh PRF. When applied in a rat craniofacial defect model for six weeks, LPRF resulted in 97% bony coverage of the defect, compared to 84% for fresh PRF, 64% for fibrin, and 16% without scaffold. Moreover, LPRF thickened the trabecular diameter by 25% when compared to fresh PRF and fibrin, and only LPRF and fresh PRF resulted in the formation of interconnected trabeculae across the defect. Together, these studies support the application of lyophilized PRF as a biomimetic scaffold for craniofacial bone regeneration and mineralized tissue engineering.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland

【 预 览 】
附件列表
Files Size Format View
RO202003190026025ZK.pdf 4757KB PDF download
  文献评价指标  
  下载次数:13次 浏览次数:18次