High Yield of Wax Ester Synthesized from Cetyl Alcohol and Octanoic Acid by Lipozyme RMIM and Novozym 435

International Journal of Molecular Sciences

Chia-Hung Kuo³  Hsin-Hung Chen⁴  Jiann-Hwa Chen¹  Yung-Chuan Liu² 

[1] Graduate Institute of Molecular Biology, National Chung Hsing University, 250 Kuo-kuang Road, Taichung, 402, Taiwan; E-Mail:;Department of Chemical Engineering, National Chung Hsing University, 250 Kuo-kuang Road, Taichung, 402, Taiwan; E-Mail:;Biotechnology Center, National Chung Hsing University, 250 Kuo-kuang Road, Taichung 402, Taiwan; E-Mail:;Department and Graduate Program of Bioindustry Technology, Dayeh University, 168 University Road, Chang-Hwa, 515, Taiwan; E-Mail:

关键词: optimization;    wax esters;    lipase;    esterification;    Rhizomucor miehei;    Candida antarctica;   

DOI  :  10.3390/ijms130911694

来源: mdpi

【 摘 要 】

Wax esters are long-chain esters that have been widely applied in premium lubricants, parting agents, antifoaming agents and cosmetics. In this study, the biocatalytic preparation of a specific wax ester, cetyl octanoate, is performed in n-hexane using two commercial immobilized lipases, i.e., Lipozyme^® RMIM (Rhizomucor miehei) and Novozym^® 435 (Candida antarctica). Response surface methodology (RSM) and 5-level-4-factor central composite rotatable design (CCRD) are employed to evaluate the effects of reaction time (1–5 h), reaction temperature (45–65 °C), substrate molar ratio (1–3:1), and enzyme amount (10%–50%) on the yield of cetyl octanoate. Using RSM to optimize the reaction, the maximum yields reached 94% and 98% using Lipozyme^® RMIM and Novozym^® 435, respectively. The optimum conditions for synthesis of cetyl octanoate by both lipases are established and compared. Novozym^® 435 proves to be a more efficient biocatalyst than Lipozyme^® RMIM.

【 授权许可】

CC BY   
© 2012 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.