Production of biodiesel catalyzed by Candida rugosa lipase at interface of w/o microemulsion system

Journal of the Brazilian Chemical Society

Liang, Xinle¹  Ren, Dahai¹  Fang, Miaoli¹  Tang, Xiujuan¹  Song, Lelian¹  Zhejiang Gongshang University, Hangzhou, P. R. China¹  Tan, Zhongqin¹  Clarkson University¹  Du, Huan¹  Han, Xiaoxiang¹  Zhejiang Medical College, Hangzhou, P. R. China¹ 

关键词: microemulsion;    lipase;    esterification;    response surface methodology (RSM);    inhibition;   

DOI  :  10.5935/0103-5053.20140165

学科分类：化学（综合）

来源: SciELO

【 摘 要 】

The synthesis between palmitic acid and methanol was carried out in a w/o reverse microemulsion prepared from the mixture of dodecylbenzenesulfonic acid (DBSA)/isooctane/water. Box-Behnken design was adopted to evaluate the effect of significant factors on the methyl palmitate yield and response surface methodology (RSM), which was employed to optimize the process parameters in the esterification. The conditions that showed optimal results for methyl palmitate preparation were: 3.33 w0 ([H2O]/[surfactant]), 4.2 h reaction time, 5:1 methanol/acid molar ratio, and 130 mg g-1 lipase ([lipase]/[acid]) concentration. The following verification experiment obtained a result of 97% in almost total agreement with the expected value (98%). The kinetic constants of the model were determined by experiments at 40 °C with initial concentrations of 0.025-0.25 mol L-1 palmitic acid and 0.025-0.3 mol L-1 methanol in the microemulsion system. The kinetic studies showed that the reaction obeyed the Ping-Pong bi-bi mechanism with inhibition by methanol.

【 授权许可】

Unknown