Biotechnological Utilisation of Fusel Oil, A Food Industry By-Product - A Kinetic Model on Enzymatic Esterification of i-Amyl Alcohol and Oleic Acid by Candida antarctica Lipase B

Food Technology and Biotechnology

卷:46

Nándor Nemestóthy¹  Erika Fehér¹  Katalin Bélafi-Bakó¹  László Gubicza¹ 

[1] Research Institute of Chemical and Process Engineering, Pannon University, Egyetem u. 2, HU-8200 Veszprém, Hungary;

关键词: fusel oil;    esterification;    lipase;    ping-pong bi-bi mechanism;   

DOI  :  

来源: DOAJ

【 摘 要 】

Fusel oil is a by-product of distilleries, its main component is i-amyl alcohol, which can form ester compounds. Esterification of oleic acid and i-amyl alcohol by Candida antarctica lipase B (Novozym 435 preparation) in n-heptane solvent was studied in this work. Ping-pong bi-bi mechanism (inhibition phenomena taken into account) was applied as a complex kinetic model. The parameters of the model were determined by numerical methods. It was found that four-parameter model fitted well with the experimental results and described properly the enzymatic reaction.

【 授权许可】

Unknown