Structural Analysis of a Heteropolysaccharide from Saccharina japonica by Electrospray Mass Spectrometry in Tandem with Collision-Induced Dissociation Tandem Mass Spectrometry (ESI-CID-MS/MS)

Marine Drugs

Weihua Jin¹  Jing Wang¹  Sumei Ren²  Ni Song² 

[1] Institute of Oceanology, Chinese Academy of Science, Qingdao 266071, China;College of Medicine and Pharmaceutics, Ocean University of China, Qingdao 266003, China;

关键词: ESI-CID-MS/MS;    fucoidan;    oligosaccharide;    Saccharina japonica;   

DOI  :  10.3390/md10102138

来源: mdpi

【 摘 要 】

A fucoidan extracted from Saccharina japonica was fractionated by anion exchange chromatography. The most complex fraction F0.5 was degraded by dilute sulphuric acid and then separated by use of an activated carbon column. Fraction Y1 was fractionated by anion exchange and gel filtration chromatography while Fraction Y2 was fractionated by gel filtration chromatography. The fractions were determined by ESI-MS and analyzed by ESI-CID-MS/MS. It was concluded that F0.5 had a backbone of alternating 4-linked GlcA and 2-linked Man with the first Man residue from the nonreducing end accidentally sulfated at C6. In addition, F0.5 had a 3-linked glucuronan, in accordance with a previous report by NMR. Some other structural characteristics included GlcA 1→3 Man 1→4 GlcA, Man 1→3 GlcA 1→4 GlcA, Fuc 1→4 GlcA and Fuc 1→3 Fuc. Finally, it was shown that fucose was sulfated at C2 or C4 while galactose was sulfated at C2, C4 or C6.

【 授权许可】

CC BY   
© 2012 by the authors; licensee MDPI, Basel, Switzerland.