Preparation of a Cu(II)-PVA/PA6 Composite Nanofibrous Membrane for Enzyme Immobilization

International Journal of Molecular Sciences

Quan Feng²  Bin Tang¹  Qufu Wei²  Dayin Hou¹  Songmei Bi¹ 

[1] Textiles and Clothing Department, Anhui Polytechnic University, Wuhu 241000, China; E-Mails:;Key Laboratory of Eco-Textiles (Ministry of Education), Jiangnan University, Wuxi 214122, China; E-Mails:

关键词: PVA/PA6;    nanofibrous membranes;    metal chelation;    enzyme;    immobilization;   

DOI  :  10.3390/ijms131012734

来源: mdpi

【 摘 要 】

PVA/PA6 composite nanofibers were formed by electrospinning. Cu(II)-PVA/PA6 metal chelated nanofibers, prepared by the reaction between PVA/PA6 composite nanofibers and Cu²⁺ solution, were used as the support for catalase immobilization. The result of the experiments showed that PVA/PA6 composite nanofibers had an excellent chelation capacity for Cu²⁺ ions, and the structures of nanofibers were stable during the reaction with Cu²⁺ solution. The adsorption of Cu(II) onto PVA/PA6 composite nanofibers was studied by the Langmuir isothermal adsorption model. The maximum amount of coordinated Cu(II) (q_m) was 3.731 mmol/g (dry fiber), and the binding constant (K_l) was 0.0593 L/mmol. Kinetic parameters were analyzed for both immobilized and free catalases. The value of V_max (3774 μmol/mg·min) for the immobilized catalases was smaller than that of the free catalases (4878 μmol/mg·min), while the K_m for the immobilized catalases was larger. The immobilized catalases showed better resistance to pH and temperature than that of free form, and the storage stabilities, reusability of immobilized catalases were significantly improved. The half-lives of free and immobilized catalases were 8 days and 24 days, respectively.

【 授权许可】

CC BY   
© 2012 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.