Laccase Immobilization by Chelated Metal Ion Coordination Chemistry

Polymers

Qingqing Wang¹  Jing Cui¹  Guohui Li¹  Jinning Zhang¹  Fenglin Huang¹ 

[1] Key Laboratory of Eco-Textiles, Ministry of Education, Jiangnan University, Wuxi 214122, China;

关键词: amidoxime polyacrylonitrile;    metal chelation;    laccase;    immobilization;    nanofibers;   

DOI  :  10.3390/polym6092357

来源: mdpi

【 摘 要 】

In this work, amidoxime polyacrylonitrile (AOPAN) nanofibrous membrane was prepared by a reaction between PAN nanofibers and hydroxylamine hydrochloride. The AOPAN nanofibrous membranes were used for four metal ions (Fe³⁺, Cu²⁺, Ni²⁺, Cd²⁺) chelation under different conditions. Further, the competition of different metal ions coordinating with AOPAN nanofibrous membrane was also studied. The AOPAN chelated with individual metal ion (Fe³⁺, Cu²⁺, Ni²⁺, Cd²⁺) and also the four mixed metal ions were further used for laccase (Lac) immobilization. Compared with free laccase, the immobilized laccase showed better resistance to pH and temperature changes as well as improved storage stability. Among the four individual metal ion chelated membranes, the stability of the immobilized enzymes generally followed the order as Fe–AOPAN–Lac > Cu–AOPAN–Lac > Ni–AOPAN–Lac > Cd–AOPAN–Lac. In addition, the immobilized enzyme on the carrier of AOPAN chelated with four mixed metal ions showed the best properties.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.