International Journal of Molecular Sciences | |
Histone Deacetylase (HDAC) Inhibitors Down-Regulate Endothelial Lineage Commitment of Umbilical Cord Blood Derived Endothelial Progenitor Cells | |
Florin Iordache1  Cosmin Buzila1  Andrei Constantinescu1  Eugen Andrei1  | |
[1] Institute of Cellular Biology and Pathology of Romanian Academy, “Nicolae Simionescu”, 8, B.P. Hasdeu, Bucharest 050568, Romania; | |
关键词: endothelial progenitor cells; differentiation; acetylation; histone deacetylases; | |
DOI : 10.3390/ijms131115074 | |
来源: mdpi | |
【 摘 要 】
To test the involvement of histone deacetylases (HDACs) activity in endothelial lineage progression, we investigated the effects of HDAC inhibitors on endothelial progenitors cells (EPCs) derived from umbilical cord blood (UCB). Adherent EPCs, that expressed the endothelial marker proteins (PCAM-1, CD105, CD133, and VEGFR2) revealed by flow cytometry were treated with three HDAC inhibitors: Butyrate (BuA), Trichostatin A (TSA), and Valproic acid (VPA). RT-PCR assay showed that HDAC inhibitors down-regulated the expression of endothelial genes such as
【 授权许可】
CC BY
© 2012 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.
【 预 览 】
Files | Size | Format | View |
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RO202003190040620ZK.pdf | 604KB | download |