Antioxidant Properties of cis-Z,Z'-3a.7a',7a.3a'-Dihydroxy-ligustilide on Human Umbilical Vein Endothelial Cells in Vitro

Molecules

Weidong Li¹  Yu Wu¹  Xuedong Liu¹  Cuiping Yan¹  Dan Liu¹  Yang Pan¹  Guangming Yang¹  Fangzhou Yin¹  Zebin Weng¹  Ding Zhao¹  Zhipeng Chen¹ 

[1] ¹College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210046, China

关键词: cis-Z;    -3a.7a'"'>Z'-3a.7a';    7a.3a'-dihydroxyligustilide;    antioxidant;    human umbilical vein endothelial cell;    cell cycle;   

DOI  :  10.3390/molecules18010520

来源: mdpi

【 摘 要 】

A new chemical component, cis-Z,Z'-3a.7a',7a.3a'-dihydroxyligustilide, was isolated from Angelica sinensis and its structure elucidated from its NMR and MS spectra and confirmed by X-ray single crystal diffraction analysis. We also explored the antioxidative properties of cis-Z,Z'-3a.7a',7a.3a'-dihydroxyligustilide on human umbilical vein endothelial cells (HUVECs) against injuries induced by hydrogen peroxide (H₂O₂) using an MTT assay and flow cytometry analysis. In addition, the activities of superoxide dismutase (SOD), malondialdehyde (MDA), lactate dehydrogenase (LDH), nitric oxide (NO) and reactive oxygen species (ROS) were determined. We found that cis-Z,Z'-3a.7a',7a.3a'-dihydroxyligustilide increased the viability of HUVECs injured by H₂O₂ in a dose-dependent manner, reduced the apoptosis of HUVEC, and enhanced HUVEC proliferation. Our results demonstrated the remarkable in vitro antioxidative activities of this compound, indicating that it could be a potential antioxidant with protective effects against H₂O₂-induced HUVEC injuries.

【 授权许可】

CC BY   
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.