Anti-Mycobacterial Activity of Marine Fungus-Derived 4-Deoxybostrycin and Nigrosporin

Molecules

Cong Wang¹  Juan Wang¹  Yuhong Huang¹  Hong Chen¹  Yan Li¹  Lili Zhong¹  Yi Chen¹  Shengping Chen¹  Jun Wang¹  Juling Kang¹  Yi Peng¹  Bin Yang¹  Yongcheng Lin¹  Zhigang She¹ 

[1] ¹Department of Microbiology, Gangdong Provincial Research Centre for Severe Infectious Disease Prevention and Control Technology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, Guangdong, China

关键词: anti-mycobacterial activity;    4-deoxybostrycin;    differentially expressed genes;   

DOI  :  10.3390/molecules18021728

来源: mdpi

【 摘 要 】

4-Deoxybostrycin is a natural anthraquinone compound isolated from the Mangrove endophytic fungus Nigrospora sp. collected from the South China Sea. Nigrosporin is the deoxy-derivative of 4-deoxybostrycin. They were tested against mycobacteria, especially Mycobacterium tuberculosis. In the Kirby-Bauer disk diffusion susceptibility test, they both had inhibition zone sizes of over 25 mm. The results of the absolute concentration susceptibility test suggested that they had inhibitory effects against mycobacteria. Moreover, 4-deoxybostrycin exhibited good inhibition which was even better than that of firstline anti-tuberculosis (TB) drugs against some clinical multidrug-resistant (MDR)M. tuberculosis strains. The gene expression profile of M. tuberculosis H37Rv after treatment with 4-deoxybostrycin was compared with untreated bacteria. One hundred and nineteen out of 3,875 genes were significantly different in M. tuberculosis exposed to 4-deoxybostrycin from control. There were 46 functionally known genes which are involved in metabolism, information storage and processing and cellular processes. The differential expressions of six genes were further confirmed by quantitative real-time polymerase chain reaction (qRT-PCR). The present study provides a useful experiment basis for exploitation of correlative new drugs against TB and for finding out new targets of anti-mycobacterial therapy.

【 授权许可】

CC BY   
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.