Transcriptome Analysis and Discovery of Genes Involved in Immune Pathways from Coelomocytes of Sea Cucumber (Apostichopus japonicus) after Vibrio splendidus Challenge

International Journal of Molecular Sciences

Qiong Gao²  Meijie Liao¹  Yingeng Wang¹  Bin Li¹  Zheng Zhang¹  Xiaojun Rong¹  Guiping Chen¹  Lan Wang¹ 

[1] Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China; E-Mails:;Fisheries College, Ocean University of China, Qingdao 266100, China; E-Mail:

关键词: sea cucumber (Apostichopus japonicus);    Vibrio splendidus;    transcriptome sequencing;    differentially expressed genes;    bacteria-resistant gene;    bacteria-susceptible gene;   

DOI  :  10.3390/ijms160716347

来源: mdpi

【 摘 要 】

Vibrio splendidus is identified as one of the major pathogenic factors for the skin ulceration syndrome in sea cucumber (Apostichopus japonicus), which has vastly limited the development of the sea cucumber culture industry. In order to screen the immune genes involving Vibrio splendidus challenge in sea cucumber and explore the molecular mechanism of this process, the related transcriptome and gene expression profiling of resistant and susceptible biotypes of sea cucumber with Vibrio splendidus challenge were collected for analysis. A total of 319,455,942 trimmed reads were obtained, which were assembled into 186,658 contigs. After that, 89,891 representative contigs (without isoform) were clustered. The analysis of the gene expression profiling identified 358 differentially expression genes (DEGs) in the bacterial-resistant group, and 102 DEGs in the bacterial-susceptible group, compared with that in control group. According to the reported references and annotation information from BLAST, GO and KEGG, 30 putative bacterial-resistant genes and 19 putative bacterial-susceptible genes were identified from DEGs. The qRT-PCR results were consistent with the RNA-Seq results. Furthermore, many DGEs were involved in immune signaling related pathways, such as Endocytosis, Lysosome, MAPK, Chemokine and the ERBB signaling pathway.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.