Expression Analysis of Immune Related Genes Identified from the Coelomocytes of Sea Cucumber (Apostichopus japonicus) in Response to LPS Challenge

International Journal of Molecular Sciences

Ying Dong¹  Hongjuan Sun¹  Zunchun Zhou¹  Aifu Yang²  Zhong Chen²  Xiaoyan Guan²  Shan Gao²  Bai Wang²  Bei Jiang²  Jingwei Jiang² 

[1] Liaoning Key Lab of Marine Fishery Molecular Biology, Liaoning Ocean and Fisheries Science Research Institute, Dalian 116023, China;

关键词: sea cucumber (Apostichopus japonicus);    differentially expressed genes;    quantitative real-time polymerase chain reaction;   

DOI  :  10.3390/ijms151119472

来源: mdpi

【 摘 要 】

The sea cucumber (Apostichopus japonicus) occupies a basal position during the evolution of deuterostomes and is also an important aquaculture species. In order to identify more immune effectors, transcriptome sequencing of A. japonicus coelomocytes in response to lipopolysaccharide (LPS) challenge was performed using the Illumina HiSeq™ 2000 platform. One hundred and seven differentially expressed genes were selected and divided into four functional categories including pathogen recognition (25 genes), reorganization of cytoskeleton (27 genes), inflammation (41 genes) and apoptosis (14 genes). They were analyzed to elucidate the mechanisms of host-pathogen interactions and downstream signaling transduction. Quantitative real-time polymerase chain reactions (qRT-PCRs) of 10 representative genes validated the accuracy and reliability of RNA sequencing results with the correlation coefficients from 0.88 to 0.98 and p-value <0.05. Expression analysis of immune-related genes after LPS challenge will be useful in understanding the immune response mechanisms of A. japonicus against pathogen invasion and developing strategies for resistant markers selection.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.