A Disposable Microfluidic Virus Concentration Device Based on Evaporation and Interfacial Tension

Diagnostics

Jane Yuqian Zhang²  Madhumita Mahalanabis²  Lena Liu²  Jessie Chang²  Nira R. Pollock¹ 

[1] Division of Infectious Diseases, Beth Israel Deaconess Medical Center and Department of Lab Medicine, Boston Children’s Hospital, 300 Longwood Ave, Boston, MA 02115, USA; E-Mail:;Department of Biomedical Engineering, Boston University, 44 Cummington Mall, Boston, MA 02215, USA; E-Mails:

关键词: microfluidic;    sample preparation;    virus;    point-of-care diagnostics;    influenza;    PCR;   

DOI  :  10.3390/diagnostics3010155

来源: mdpi

【 摘 要 】

We report a disposable and highly effective polymeric microfluidic viral sample concentration device capable of increasing the concentration of virus in a human nasopharyngeal specimen more than one order of magnitude in less than 30 min without the use of a centrifuge. The device is fabricated using 3D maskless xurography method using commercially available polymeric materials, which require no cleanroom operations. The disposable components can be fabricated and assembled in five minutes. The device can concentrate a few milliliters (mL) of influenza virus in solution from tissue culture or clinical nasopharyngeal swab specimens, via reduction of the fluid volume, to tens of microliters μL). The performance of the device was evaluated by nucleic acid extraction from the concentrated samples, followed by a real-time quantitative polymerase chain reaction (qRT-PCR). The viral RNA concentration in each sample was increased on average over 10-fold for both cultured and patient specimens compared to the starting samples, with recovery efficiencies above 60% for all input concentrations. Highly concentrated samples in small fluid volumes can increase the downstream process speed of on-chip nucleic acid extraction, and result in improvements in the sensitivity of many diagnostic platforms that interrogate small sample volumes.

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland.