A Portable Droplet Magnetofluidic Device for Point-of-Care Detection of Multidrug-Resistant Candida auris

Frontiers in Bioengineering and Biotechnology

Kushagra Shah¹  Fan-En Chen¹  Alexander Y. Trick¹  Mei Jin¹  Liben Chen²  Kuangwen Hsieh²  Pei-Wei Lee²  Hoan Thanh Ngo²  Sean X. Zhang³  Marissa Totten³  Tza-Huei Wang⁴ 

[1] Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD, United States;Department of Mechanical Engineering, Johns Hopkins University, Baltimore, MD, United States;Division of Microbiology, Department of Pathology, Johns Hopkins School of Medicine, Baltimore, MD, United States;Institute of NanoBioTechnology, Johns Hopkins University, Baltimore, MD, United States;

关键词: droplet magnetofluidics;    point-of-care diagnostics;    sample preparation;    lysis;    miniaturized rapid PCR;    Candida auris;   

DOI  :  10.3389/fbioe.2022.826694

来源: DOAJ

【 摘 要 】

Candida auris is an emerging multidrug-resistant fungal pathogen that can cause severe and deadly infections. To date, C. auris has spurred outbreaks in healthcare settings in thirty-three countries across five continents. To control and potentially prevent its spread, there is an urgent need for point-of-care (POC) diagnostics that can rapidly screen patients, close patient contacts, and surveil environmental sources. Droplet magnetofluidics (DM), which leverages nucleic acid-binding magnetic beads for realizing POC-amenable nucleic acid detection platforms, offers a promising solution. Herein, we report the first DM device—coined POC.auris—for POC detection of C. auris. As part of POC.auris, we have incorporated a handheld cell lysis module that lyses C. auris cells with 2 min hands-on time. Subsequently, within the palm-sized and automated DM device, C. auris and control DNA are magnetically extracted and purified by a motorized magnetic arm and finally amplified via a duplex real-time quantitative PCR assay by a miniaturized rapid PCR module and a miniaturized fluorescence detector—all in ≤30 min. For demonstration, we use POC.auris to detect C. auris isolates from 3 major clades, with no cross reactivity against other Candida species and a limit of detection of ∼300 colony forming units per mL. Taken together, POC.auris presents a potentially useful tool for combating C. auris.

【 授权许可】

Unknown