The Ameliorative Effects of l-2-Oxothiazolidine-4-Carboxylate on Acetaminophen-Induced Hepatotoxicity in Mice

Molecules

Jiwon Choi³  Kwang-Hyun Park⁴  Sung Zoo Kim¹  Jun Ho Shin² 

[1] Department of Physiology, School of Medicine, Chonbuk National University, Jeonju 561-803, Korea; E-Mail:;Ato Q&A Incorperation, Jeonju 560-759, Korea; E-Mail:;Department of Radiological Sciences, Jeonju University, Jeonju 560-759, Korea; E-Mail:;Department of Oriental Pharmaceutical Development, Nambu University, Gwangju 506-706, Korea; E-Mail:

关键词: l-2-oxothiazolidine-4-carboxylate;    acetaminophen-induced hepatotoxicity;    apoptosis;    oxidative stress;    antioxidant;   

DOI  :  10.3390/molecules18033467

来源: mdpi

【 摘 要 】

The aim of the study was to investigate the ameliorative effects and the mechanism of action of l-2-oxothiazolidine-4-carboxylate (OTC) on acetaminophen (APAP)-induced hepatotoxicity in mice. Mice were randomly divided into six groups: normal control group, APAP only treated group, APAP + 25 mg/kg OTC, APAP + 50 mg/kg OTC, APAP + 100 mg/kg OTC, and APAP + 100 mg/kg N-acetylcysteine (NAC) as a reference control group. OTC treatment significantly reduced serum alanine aminotransferase and aspartate aminotransferase levels in a dose dependent manner. OTC treatment was markedly increased glutathione (GSH) production and glutathione peroxidase (GSH-px) activity in a dose dependent manner. The contents of malondialdehyde and 4-hydroxynonenal in liver tissues were significantly decreased by administration of OTC and the inhibitory effect of OTC was similar to that of NAC. Moreover, OTC treatment on APAP-induced hepatotoxicity significantly reduced the formation of nitrotyrosin and terminal deoxynucleotidyl transferase dUTP nick end labeling positive areas of liver tissues in a dose dependent manner. Furthermore, the activity of caspase-3 in liver tissues was reduced by administration of OTC in a dose dependent manner. The ameliorative effects of OTC on APAP-induced liver damage in mice was similar to that of NAC. These results suggest that OTC has ameliorative effects on APAP-induced hepatotoxicity in mice through anti-oxidative stress and anti-apoptotic processes.

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland.