α-Glucosidase Inhibitory Effect and Simultaneous Quantification of Three Major Flavonoid Glycosides in Microctis folium

Molecules

Yan-Gan Chen²  Ping Li²  Peng Li²  Ru Yan²  Xiao-Qi Zhang¹  Ying Wang¹  Xian-Tao Zhang³  Wen-Cai Ye¹ 

[1] Institute of Traditional Chinese Medicine and Natural Products, Jinan University, Guangzhou 510632, China; E-Mails:;State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, China; E-Mails:;Guangdong Research Institute of Traditional Chinese Medicine, Guangzhou 510520, China; E-Mail:

关键词: Microctis Folium;    flavonoid glycosides;    α-glucosidase inhibitory effect;    HPLC-DAD;    quantification;   

DOI  :  10.3390/molecules18044221

来源: mdpi

【 摘 要 】

Microctis Folium, the leaves of Microcos paniculata L., is a commonly used herbal tea material. The methanol extract of Microctis Folium and its principle compounds vitexin (1), isovitexin (2) and isorhamnetin 3-O-β-d-rutinoside (3) were investigated for their α-glucosidase inhibitory effects. The extract showed strong α-glucosidase inhibitory effect (IC₅₀ = 61.30 μg/mL) and the three flavonoid glycosides 1–3 exerted satisfactory α-glucosidase inhibitory effects, with IC₅₀ values of 244.0 μM, 266.2 μM and 275.4 μM, respectively. A simple and reliable HPLC-DAD method was developed for the quantification of the three flavonoid glycosides in Microctis Folium and applied successfully to determine contents of these components in samples collected from different locations. This study suggested that Microctis Folium may be a promising candidate for development of herbal antidiabetes drugs, and vitexin, isovitexin and isorhamnetin 3-O-β-d-rutinoside can be the biomarkers and chemical markers for this plant substance.

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland.