Anti-Infective Potential of Marine Invertebrates and Seaweeds from the Brazilian Coast

Molecules

Éverson Miguel Bianco²  Simone Quintana de Oliveira²  Caroline Rigotto¹  Maiko Luis Tonini⁴  Tatiana da Rosa Guimar฾s²  Francine Bittencourt²  Lidiane Pires Gouvສ⁵  Cassandra Aresi²  Maria Tereza Rojo de Almeida²  Maria Izabel Goularte Moritz²  Cintia Dalcuche Leal Martins⁵  Fernando Scherner⁵  João Luís Carraro³  Paulo Antunes Horta⁵  Flávio Henrique Reginatto²  Mario Steindel⁴  Cláudia Maria Oliveira Simཞs¹ 

[1] Laboratório de Virologia Aplicada, Departamento de Ciências Farmacêuticas, Universidade Federal de Santa Catarina, CEP 88.040-900, Florianópolis, SC, Brasil; E-Mails:;Laboratório de Produtos Naturais, Departamento de Ciências Farmacêuticas, Universidade Federal de Santa Catarina, CEP 88.040-900, Florianópolis, SC, Brasil; E-Mails:;Programa de Pós-graduação em Ecologia, Universidade Federal do Rio Grande do Sul, CEP 91.501-970, Porto Alegre, RS, Brasil; E-Mail:;Laboratório de Protozoologia, Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de Santa Catarina, CEP 88.040-900, Florianópolis, SC, Brasil; E-Mails:;Laboratório de Ficologia, Departamento de Botânica, Universidade Federal de Santa Catarina, CEP 88.040-900, Florianópolis, SC, Brasil; E-Mails:

关键词: marine natural products;    seaweeds;    marine invertebrates;    antileishmanial activity;    antitrypanosomal activity;    antimicrobial activity;    anti-HSV-1 activity;   

DOI  :  10.3390/molecules18055761

来源: mdpi

【 摘 要 】

This manuscript describes the evaluation of anti-infective potential in vitro of organic extracts from nine sponges, one ascidian, two octocorals, one bryozoan, and 27 seaweed species collected along the Brazilian coast. Antimicrobial activity was tested against Staphylococcus aureus (ATCC 25923), Enterococcus faecalis (ATCC 29212), Pseudomonasaeruginosa (ATCC 27853), Escherichia coli (ATCC 25922) and Candida albicans (ATCC 10231) by the disk diffusion method. Antiprotozoal activity was evaluated against Leishmania braziliensis (MHOM/BR/96/LSC96-H3) promastigotes and Trypanosomacruzi (MHOM/BR/00/Y) epimastigotes by MTT assay. Activity against intracellular amastigotes of T. cruzi and L. brasiliensis in murine macrophages was also evaluated. Antiviral activity was tested against Herpes Simplex Virus type 1 (HSV-1, KOS strain) by the plaque number reduction assay (IC₅₀). Cytotoxicity on VERO cells was evaluated by the MTT assay (CC₅₀). The results were expressed as SI = CC₅₀/IC₅₀. The most promising antimicrobial results were obtained against S. aureus and C. albicans with Dragmacidon reticulatum. Among the seaweeds, only Osmundaria obtusiloba showed moderate activity against P. aeruginosa. Concerning antiprotozoal activity, Bugula neritina, Carijoa riseii, Dragmaxia anomala and Haliclona (Halichoclona) sp. showed the most interesting results, mainly against extracellular promastigote forms of L. braziliensis (66, 35.9, 97.2, and 43.6% inhibition, respectively). Moreover, six species of seaweeds Anadyomene saldanhae, Caulerpacupressoides, Canistrocarpus cervicornis, Dictyota sp., Ochtodes secundiramea, and Padina sp. showed promising results against L. braziliensis (87.9, 51.7, 85.9, 93.3, 99.7, and 80.9% inhibition, respectively), and only Dictyota sp. was effective against T. cruzi (60.4% inhibition). Finally, the antiherpes activity was also evaluated, with Haliclona (Halichoclona)sp. and Petromica citrina showing the best results (SI = 11.9 and SI > 5, respectively). All the active extracts deserve special attention in further studies to chemically characterize the bioactive compounds, and to perform more refined biological assays.

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland.