Preparation of Novel Poly(hydroxyethyl methacrylate-co-glycidyl methacrylate)-Grafted Core-Shell Magnetic Chitosan Microspheres and Immobilization of Lactase

International Journal of Molecular Sciences

Wei Zhao²  Rui-Jin Yang¹  Ting-Ting Qian¹  Xiao Hua²  Wen-Bin Zhang² 

[1] School of Food Science and Technology, Jiangnan University, No. 1800 Lihu Road, Wuxi 214122, China; E-Mail:;State Key Laboratory of Food Science and Technology, Jiangnan University, No. 1800 Lihu Road, Wuxi 214122, China; E-Mails:

关键词: magnetic chitosan microspheres;    reversed-phase suspension polymerization;    immobilization;    lactase;   

DOI  :  10.3390/ijms140612073

来源: mdpi

【 摘 要 】

Poly(hydroxyethyl methacrylate-co-glycidyl methacrylate)-grafted magnetic chitosan microspheres (HG-MCM) were prepared using reversed-phase suspension polymerization method. The HG-MCM presented a core-shell structure and regular spherical shape with poly(hydroxyethyl methacrylate-co-glycidyl methacrylate) grafted onto the chitosan layer coating the Fe₃O₄ cores. The average diameter of the magnetic microspheres was 10.67 μm, within a narrow size distribution of 6.6–17.4 μm. The saturation magnetization and retentivity of the magnetic microspheres were 7.0033 emu/g and 0.6273 emu/g, respectively. The application of HG-MCM in immobilization of lactase showed that the immobilized enzyme presented higher storage, pH and thermal stability compared to the free enzyme. This indicates that HG-MCM have potential applications in bio-macromolecule immobilization.

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland