Hydrolysis of whey lactose by immobilized β-Galactosidase

Brazilian Archives of Biology and Technology

Marcela Panaro Mariotti¹  Hideko Yamanaka¹  Angela Regina Araujo¹  Henrique Celso Trevisan¹ 

[1] ,Unesp Instituto de Química Departamento de Bioquímica e Tecnologia QuímicaAraraquara SP ,Brasil

关键词: β-galactosidase;    immobilization;    lactase;    whey;    lactose;    hydrolysis;   

DOI  :  10.1590/S1516-89132008000600019

来源: SciELO

【 摘 要 】

Hydrolysis of whey lactose to glucose and galactose by immobilized galactosidase comes as an alternative to enlarge the possibilities of commercial use of this feedstock. To be applied at industrial scale, the process should be performed continuously .This work aimed to study the hydrolysis of whey lactose by an immobilized enzyme reactor. b-Galactosidase from Aspergillus oryzae was immobilized on silica and activity and stability were evaluated. The best immobilization results were attained by using glutaraldehyde as support's activator and enzyme stabilizer. The optimized enzyme proportion for immobilization was 15-20 mg g-1 of support. Treatments of whey were performed (microfiltration, thermal treatment and ultrafiltration), seeking the elimination of sludge, and the effects on operating the fixed bed reactor were evaluated. Ultrafiltration was the best treatment towards a proper substrate solution for feeding the reactor.

【 授权许可】

CC BY-NC   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License