期刊论文详细信息
International Journal of Molecular Sciences
Cadmium Modifies the Cell Cycle and Apoptotic Profiles of Human Breast Cancer Cells Treated with 5-Fluorouracil
Yolande Asara1  Juan A. Marchal2  Esther Carrasco2  Houria Boulaiz2  Giuliana Solinas1  Pasquale Bandiera1  Maria A. Garcia3  Cristiano Farace1  Andrea Montella1 
[1] Department of Biomedical Sciences, University of Sassari, Viale San Pietro 43/B, Sassari 07100, Italy; E-Mails:;Biopathology and Regenerative Medicine Institute (IBIMER), Centre for Biomedical Research, University of Granada, Granada E-18100, Spain; E-Mails:;Oncology Department, University Hospital “Virgen de las Nieves”, Granada E-18100, Spain; E-Mail:
关键词: cadmium;    5-fluorouracil;    MCF-7;    breast cancer;   
DOI  :  10.3390/ijms140816600
来源: mdpi
PDF
【 摘 要 】

Industrialisation, the proximity of factories to cities, and human work activities have led to a disproportionate use of substances containing heavy metals, such as cadmium (Cd), which may have deleterious effects on human health. Carcinogenic effects of Cd and its relationship with breast cancer, among other tumours, have been reported. 5-Fluorouracil (5-FU) is a fluoropyrimidine anticancer drug used to treat solid tumours of the colon, breast, stomach, liver, and pancreas. The purpose of this work was to study the effects of Cd on cell cycle, apoptosis, and gene and protein expression in MCF-7 breast cancer cells treated with 5-FU. Cd altered the cell cycle profile, and its effects were greater when used either alone or in combination with 5-FU compared with 5-FU alone. Cd significantly suppressed apoptosis of MCF-7 cells pre-treated with 5-FU. Regarding gene and protein expression, bcl2 expression was mainly upregulated by all treatments involving Cd. The expression of caspase 8 and caspase 9 was decreased by most of the treatments and at all times evaluated. C-myc expression was increased by all treatments involving Cd, especially 5-FU plus Cd at the half time of treatment. Cd plus 5-FU decreased cyclin D1 and increased cyclin A1 expression. In conclusion, our results indicate that exposure to Cd blocks the anticancer effects of 5-FU in MCF-7 cells. These results could have important clinical implications in patients treated with 5-FU-based therapies and who are exposed to high levels of Cd.

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland

【 预 览 】
附件列表
Files Size Format View
RO202003190034011ZK.pdf 779KB PDF download
  文献评价指标  
  下载次数:7次 浏览次数:8次