International Journal of Clinical and Experimental Medicine | |
MiR-300 regulate the malignancy of breast cancer by targeting p53 | |
Yan-Qiu Song1  Hui Feng1  Xiao-Heng Xu1  Hong-Mei Chen1  Da-Wei Li1  | |
关键词: MiR-300; p53; breast cancer; MCF-7; target therapy; | |
DOI : | |
学科分类:医学(综合) | |
来源: e-Century Publishing Corporation | |
【 摘 要 】
Objective: In this study, we investigated the role of miR-300 in regulating cell proliferation and invasion of breast cancer (BC) cells. Methods: MicroRNA and protein expression patterns were compared between breast cancer tissue and normal tissue and between two different prognostic groups. The up-regulation of miR-300 was confirmed by real-time reverse transcription polymerase chain reaction and its expression was analyzed in MCF-7 breast cancer cells. Results: We observed that miR-300 expression was frequently and dramatically up-regulated in human breast cancer tissues and cell lines compared with the matched adjacent normal tissues and cells. We further showed that transient and stable over-expression of miR-300 could promote cell proliferation and cell cycle progression. Moreover, p53, a key inhibitor of cell cycle, was verified as a direct target of miR-300, suggesting that miR-300 might promote breast cancer cell proliferation and invasion by regulating p53 expression. Conclusion: Our findings indicated that miR-300 up-regulation might exert some sort of antagonistic function by targeting p53 in breast cancer cell proliferation during breast tumorigenesis.
【 授权许可】
Unknown
【 预 览 】
Files | Size | Format | View |
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RO201912140865401ZK.pdf | 1229KB | download |