Epigonal Conditioned Media from Bonnethead Shark, Sphyrna tiburo, Induces Apoptosis in a T-Cell Leukemia Cell Line, Jurkat E6-1

Marine Drugs

Catherine J. Walsh¹  Carl A. Luer²  Jennifer E. Yordy¹  Theresa Cantu¹  Jodi Miedema¹  Stephanie R. Leggett¹  Brittany Leigh¹  Philip Adams¹  Marissa Ciesla¹  Courtney Bennett¹ 

[1] Marine Immunology Program, Mote Marine Laboratory, 1600 Ken Thompson Parkway, Sarasota, FL 34236, USA;Marine Biomedical Program, Mote Marine Laboratory, 1600 Ken Thompson Parkway, Sarasota, FL 34236, USA; E-Mails:

关键词: epigonal organ;    bonnethead shark;    Jurkat;    apoptosis;    tumor cell line;   

DOI  :  10.3390/md11093224

来源: mdpi

【 摘 要 】

Representatives of Subclass Elasmobranchii are cartilaginous fish whose members include sharks, skates, and rays. Because of their unique phylogenetic position of being the most primitive group of vertebrates to possess all the components necessary for an adaptive immune system, the immune regulatory compounds they possess may represent the earliest evolutionary forms of novel compounds with the potential for innovative therapeutic applications. Conditioned medium, generated from short term culture of cells from the epigonal organ of bonnethead sharks (Sphyrna tiburo), has been shown to have potent reproducible cytotoxic activity against a variety of human tumor cell lines in vitro. Existing data suggest that epigonal conditioned medium (ECM) exerts this cytotoxic activity through induction of apoptosis in target cells. This manuscript describes apoptosis induction in a representative tumor cell line, Jurkat E6-1, in response to treatment with ECM at concentrations of 1 and 2 mg/mL. Data indicate that ECM exposure initiates the mitochondrial pathway of apoptosis through activation of caspase enzymes. Future purification of ECM components may result in the isolation of an immune-regulatory compound with potential therapeutic benefit for treatment of human cancer.

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland.