Degradation of Bacterial Quorum Sensing Signaling Molecules by the Microscopic Yeast Trichosporon loubieri Isolated from Tropical Wetland Waters

Sensors

Cheng-Siang Wong²  Chong-Lek Koh¹  Choon-Kook Sam¹  Jian Woon Chen²  Yee Meng Chong²  Wai-Fong Yin² 

[1] Natural Sciences and Science Education AG, National Institute of Education, Nanyang Technological University, 1 Nanyang Walk, Singapore 637616, Singapore; E-Mails:;Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur 50603, Malaysia; E-Mails:

关键词: basidiomycetous;    biosensor;    lactonase;    N-acylhomoserine lactone;    quorum sensing;    quorum quenching;    Rapid Resolution Liquid Chromatography;    Trichosporon loubieri;    yeast;   

DOI  :  10.3390/s131012943

来源: mdpi

【 摘 要 】

Proteobacteria produce N-acylhomoserine lactones as signaling molecules, which will bind to their cognate receptor and activate quorum sensing-mediated phenotypes in a population-dependent manner. Although quorum sensing signaling molecules can be degraded by bacteria or fungi, there is no reported work on the degradation of such molecules by basidiomycetous yeast. By using a minimal growth medium containing N-3-oxohexanoylhomoserine lactone as the sole source of carbon, a wetland water sample from Malaysia was enriched for microbial strains that can degrade N-acylhomoserine lactones, and consequently, a basidiomycetous yeast strain WW1C was isolated. Morphological phenotype and molecular analyses confirmed that WW1C was a strain of Trichosporon loubieri. We showed that WW1C degraded AHLs with N-acyl side chains ranging from 4 to 10 carbons in length, with or without oxo group substitutions at the C3 position. Re-lactonisation bioassays revealed that WW1C degraded AHLs via a lactonase activity. To the best of our knowledge, this is the first report of degradation of N-acyl-homoserine lactones and utilization of N-3-oxohexanoylhomoserine as carbon and nitrogen source for growth by basidiomycetous yeast from tropical wetland water; and the degradation of bacterial quorum sensing molecules by an eukaryotic yeast.

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland.