Quorum Quenching Bacillus sonorensis Isolated from Soya Sauce Fermentation Brine

Sensors

Wai-Fong Yin¹  Hun-Jiat Tung¹  Choon-Kook Sam²  Chong-Lek Koh² 

[1] Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia; E-Mails:;Natural Sciences and Science Education AG, National Institute of Education, Nanyang Technological University, 1 Nanyang Walk, Singapore 637616, Singapore; E-Mails:

关键词: Bacillus sonorensis;    N-acylhomoserine lactone;    quorum quenching;    quorum sensing;    soya sauce fermentation brine;   

DOI  :  10.3390/s120404065

来源: mdpi

【 摘 要 】

An N-acylhomoserine lactone (AHL)-degrading bacterial strain, L62, was isolated from a sample of fermentation brine of Chinese soya sauce by using rich medium agar supplemented with soya sauce (10% v/v). L62, a rod-shaped Gram positive bacterium with amylolytic activity, was phylogentically related to Bacillus sonorensis by 16S ribosomal DNA and rpoB sequence analyses. B. sonorensis L62 efficiently degraded N-3-oxohexanoyl homoserine lactone and N-octanoylhomoserine lactone. However, the aiiA homologue, encoding an autoinducer inactivation enzyme catalyzing the degradation of AHLs, was not detected in L62, suggesting the presence of a different AHL-degrading gene in L62. To the best of our knowledge, this is the first report of AHL-degrading B. sonorensis from soya sauce liquid state fermentation.

【 授权许可】

CC BY   
© 2012 by the authors; licensee MDPI, Basel, Switzerland.