OsPOP5, A Prolyl Oligopeptidase Family Gene from Rice Confers Abiotic Stress Tolerance in Escherichia coli

International Journal of Molecular Sciences

Cun-Mei Tan¹  Rong-Jun Chen¹  Jian-Hua Zhang¹  Xiao-Ling Gao²  Li-Hua Li²  Ping-Rong Wang²  Xiao-Jian Deng² 

[1] Rice Institute, Sichuan Agriculture of University, Chengdu 611130, China;

关键词: prolyl oligopeptidase;    abiotic stress;    protein expression;    E. coli;    Oryza sativa L;   

DOI  :  10.3390/ijms141020204

来源: mdpi

【 摘 要 】

The prolyl oligopeptidase family, which is a group of serine peptidases, can hydrolyze peptides smaller than 30 residues. The prolyl oligopeptidase family in plants includes four members, which are prolyl oligopeptidase (POP, EC3.4.21.26), dipeptidyl peptidase IV (DPPIV, EC3.4.14.5), oligopeptidase B (OPB, EC3.4.21.83), and acylaminoacyl peptidase (ACPH, EC3.4.19.1). POP is found in human and rat, and plays important roles in multiple biological processes, such as protein secretion, maturation and degradation of peptide hormones, and neuropathies, signal transduction and memory and learning. However, the function of POP is unclear in plants. In order to study POP function in plants, we cloned the cDNA of the OsPOP5 gene from rice by nested-PCR. Sequence analysis showed that the cDNA encodes a protein of 596 amino acid residues with M_w ≈ 67.29 kD. In order to analyze the protein function under different abiotic stresses, OsPOP5 was expressed in Escherichia coli. OsPOP5 protein enhanced the tolerance of E. coli to high salinity, high temperature and simulated drought. The results indicate that OsPOP5 is a stress-related gene in rice and it may play an important role in plant tolerance to abiotic stress.

【 授权许可】

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