Transcriptional Profiling of Hilar Nodes from Pigs after Experimental Infection with Actinobacillus Pleuropneumoniae

International Journal of Molecular Sciences

Shumin Yu²  Zhicai Zuo²  Hengmin Cui²  Mingzhou Li³  Xi Peng²  Ling Zhu²  Ming Zhang³  Xuewei Li³  Zhiwen Xu²  Meng Gan²  Junliang Deng²  Jing Fang²  Jideng Ma³  Shengqun Su¹  Ya Wang²  Liuhong Shen²  Xiaoping Ma²  Zhihua Ren²  Bangyuan Wu² 

[1] Library of Sichuan Agricultural University, Ya’an 625014, China; E-Mail:;College of Veterinary Medicine, Sichuan Agricultural University, Ya’an 625014, China; E-Mails:;College of Animal Science and Technology, Sichuan Agricultural University, Ya’an 625014, China; E-Mails:

关键词: porcine pleuropneumonia;    infection;    Actinobacillus pleuropneumoniae;    Agilent Porcine Genechip;    microarray analyses;    cytokine;    host defense response;   

DOI  :  10.3390/ijms141223516

来源: mdpi

【 摘 要 】

The gram-negative bacterium Actinobacillus pleuropneumoniae (APP) is an inhabitant of the porcine upper respiratory tract and the causative agent of porcine pleuropneumonia (PP). In recent years, knowledge about the proinflammatory cytokine and chemokine gene expression that occurs in lung and lymph node of the APP-infected swine has been advanced. However, systematic gene expression profiles on hilar nodes from pigs after infection with Actinobacillus pleuropneumoniae have not yet been reported. The transcriptional responses were studied in hilar nodes (HN) from swine experimentally infected with APP and the control groupusing Agilent Porcine Genechip, including 43,603 probe sets. 9,517 transcripts were identified as differentially expressed (DE) at the p ≤ 0.01 level by comparing the log2 (normalized signal) of the two groups named treatment group (TG) and controls (CG). Eight hundred and fifteen of these DE transcripts were annotated as pig genes in the GenBank database (DB). Two hundred and seventy-two biological process categories (BP), 75 cellular components and 171 molecular functions were substantially altered in the TG compared to CG. Many BP were involved in host immune responses (i.e., signaling, signal transmission, signal transduction, response to stimulus, oxidation reduction, response to stress, immune system process, signaling pathway, immune response, cell surface receptor linked signaling pathway). Seven DE gene pathways (VEGF signaling pathway, Long-term potentiation, Ribosome, Asthma, Allograft rejection, Type I diabetes mellitus and Cardiac muscle contraction) and statistically significant associations with host responses were affected. Many cytokines (including NRAS, PI3K, MAPK14, CaM, HSP27, protein phosphatase 3, catalytic subunit and alpha isoform), mediating the proliferation and migration of endothelial cells and promoting survival and vascular permeability, were activated in TG, whilst many immunomodulatory cytokines were suppressed. The significant changes in the expression patterns of the genes, GO terms, and pathways, led to a decrease of antigenic peptides with antigen presenting cells presented to T lymphocytes via the major histocompatibility complex, and alleviated immune response induced APP of HN. The immune response ability of HN in the APP-infected pigs was weakened; however, cell proliferation and migration ability was enhanced.

【 授权许可】

CC BY   
© 2013 by the authors; licensee MDPI, Basel, Switzerland