Immunological study of an attenuated Salmonella Typhimurium expressing ApxIA, ApxIIA, ApxIIIA and OmpA of Actinobacillus pleuropneumoniae in a mouse model

Journal of Veterinary Medical Science

Jin HUR¹  John Hwa LEE¹  Sang-Youel PARK¹  Seong Kug EO¹  Yoonyoung CHOI¹ 

[1] Department of Bioactive Material Sciences, and Department of Veterinary Public Health, College of Veterinary Medicine, Chonbuk National University, Jeonju 561�?756, South Korea

关键词: Actinobacillus pleuropneumoniae;    porcine pleuropneumonia;    Salmonella Typhimurium mutant;   

DOI  :  10.1292/jvms.14-0428

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(20)Salmonella Typhimurium strain expressing the Actinobacillus pleuropneumoniae antigens, ApxIA, ApxIIA, ApxIIIA and OmpA, was previously constructed as a vaccine candidate for porcine pleuropneumonia. This strain was a live attenuated (∆lon∆cpxR∆asd)Salmonella as a delivery host and contained a vector containing asd. An immunological study of lymphocyte proliferation, T-lymphocyte subsets and cytokines in the splenocytes of a mouse model was carried out after stimulation with the candidate Salmonella Typhimurium by intranasal inoculation. The splenic lymphocyte proliferation and the levels of IL-4, IL-6 and IL-12 of the inoculated mice were significantly increased, and the T- and B-cell populations were also elevated. Collectively, the candidate may efficiently induce the Th1- and Th2-type immune responses.

【 授权许可】

Unknown