Development of an Antigen-DNAzyme Based Probe for a Direct Antibody-Antigen Assay Using the Intrinsic DNAzyme Activity of a Daunomycin Aptamer

Sensors

Noorsharmimi Omar¹  Qiuting Loh¹  Gee Jun Tye¹  Yee Siew Choong¹  Rahmah Noordin¹  Jörn Glökler² 

[1] Institute for Research in Molecular Medicine, Universiti Sains Malaysia, Minden 11800, Penang, Malaysia; E-Mails:;Department of Molecular Biotechnology and Functional Genomics, Technical University of Applied Sciences Wildau, Bahnhofstr. 1, Wildau 15745, Germany; E-Mail:

关键词: G-quadruplex;    DNAzyme;    gold nanoparticles;    antibody;   

DOI  :  10.3390/s140100346

来源: mdpi

【 摘 要 】

G-Quadruplex (G-4) structures are formed when G-rich DNA sequences fold into intra- or intermolecular four-stranded structures in the presence of metal ions. G-4-hemin complexes are often effective peroxidase-mimicking DNAzymes that are applied in many detection systems. This work reports the application of a G-rich daunomycin-specific aptamer for the development of an antibody-antigen detection assay. We investigated the ability of the daunomycin aptamer to efficiently catalyze the hemin-dependent peroxidase activity independent of daunomycin. A reporter probe consisting of biotinylated antigen and daunomycin aptamer coupled to streptavidin gold nanoparticles was successfully used to generate a colorimetric readout. In conclusion, the daunomycin aptamer can function as a robust alternative DNAzyme for the development of colorimetric assays.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.