Development of an Antigen-DNAzyme Based Probe for a Direct Antibody-Antigen Assay Using the Intrinsic DNAzyme Activity of a Daunomycin Aptamer

Sensors

卷:14

Jörn Glökler¹  Theam Soon Lim²  Qiuting Loh²  Gee Jun Tye²  Yee Siew Choong²  Noorsharmimi Omar²  Rahmah Noordin² 

[1] Department of Molecular Biotechnology and Functional Genomics, Technical University of Applied Sciences Wildau, Bahnhofstr. 1, Wildau 15745, Germany;

[2] Institute for Research in Molecular Medicine, Universiti Sains Malaysia, Minden 11800, Penang, Malaysia;

关键词: G-quadruplex;    DNAzyme;    gold nanoparticles;    antibody;   

DOI  :  10.3390/s140100346

来源: DOAJ

【 摘 要 】

G-Quadruplex (G-4) structures are formed when G-rich DNA sequences fold into intra- or intermolecular four-stranded structures in the presence of metal ions.G-4-hemin complexes are often effective peroxidase-mimicking DNAzymes that are applied in many detection systems. This work reports the application of a G-rich daunomycin-specific aptamer for the development of an antibody-antigen detectionassay. We investigated the ability of the daunomycin aptamer to efficiently catalyze thehemin-dependent peroxidase activity independent of daunomycin. A reporter probe consisting of biotinylated antigen and daunomycin aptamer coupled to streptavidin gold nanoparticles was successfully used to generate a colorimetric readout. In conclusion, the daunomycin aptamer can function as a robust alternative DNAzyme for the development of colorimetric assays.

【 授权许可】

Unknown