Description of an Advantageous Optical Label-Free Biosensing Interferometric Read-Out Method to Measure Biological Species

Sensors

Miguel Holgado¹  Francisco J. Sanza¹  Ana López¹  Álvaro Lavín¹  Rafael Casquel¹ 

[1] Micro-nano Photonics and Biophotonics Group, Centro Láser and Centro de Tecnología Biomédica, Universidad Politécnica de Madrid, Av. Ramiro de Maeztu 7, Madrid 28040, Spain; E-Mails:

关键词: photonic biosensors;    label-free;    rotavirus;    optical read-out;    environmental monitoring;    health monitoring;   

DOI  :  10.3390/s140203675

来源: mdpi

【 摘 要 】

In this article we report a new, simple, and reliable optical read-out detection method able to assess Rotavirus present in human sera as well as in the viral pollution sources. It is based on the interference of two interferometers used as biophotonic transducers. The method significantly improves the optical label-free biosensing response measuring both, the concentration of the AgR and its corresponding size. Two different immunoassays were carried out: Bovine Serum Albumin (BSA), and the recognition by its antibody (anti-BSA); and Rotavirus (AgR) and the recognition by its antibody (anti-AgR). In the cases studied, and using as model interferometer a simple Fabry-Perot transducer, we demonstrate a biosensing enhancement of two orders of magnitude in the Limit of Detection (LoD). In fact, this read-out optical method may have significant implications to enhance other optical label-free photonic transducers reported in the scientific literature.

【 授权许可】

CC BY   
© 2014 by the authors; licensee MDPI, Basel, Switzerland.