A Label-Free Electronic Biosensor for Detection of Bone Turnover Markers

Sensors

Yeo-Heung Yun³  Amit Bhattacharya²  Nelson B. Watts¹ 

[1] College of Medicine, University of Cincinnati Bone Health and Osteoporosis Center, 222, Piedmont Avenue, Suite 6300, Cincinnati, OH 45219; E-Mail:;Environmental Health, College of Medicine, University of Cincinnati, Cincinnati, OH 45221, USA; E-Mail:;Nanoworld and Smart Materials and Devices Laboratory, College of Engineering, University of Cincinnati, Cincinnati, OH 45221, USA; E-Mail:

关键词: immunosensor;    label-free;    electrochemical impedance;    bone turnover marker;   

DOI  :  10.3390/s91007957

来源: mdpi

【 摘 要 】

This paper describes the development of a biosensor based on label-free immunosensing for the detection of the C-terminal telopeptide bone turnover marker from type-1 collagen. A self-assembled monolayer (SAM) of dithiodipropionic acid was deposited on a gold electrode. Then streptavidin and biotinylated anti-human C-terminal telopeptide antibody were successively conjugated on the self-assembled monolayer. Electrochemical impedance measurements were made to characterize each step of the SAM/streptavidin/biotinylated antibody binding. Subsequently, electrochemical impedance was measured with different concentrations of C-teminal telopeptide. A detection limit of 50 ng/mL and a dynamic range up to 3 μg/mL were achieved. To our knowledge, this is the first attempt to develop a label-free immunosensor based on electrochemical impedance with DC bias for detection of bone-related degradation and rebuilding products. The electronic biosensor might eventually be used for quantitative point-of-care screening of bone health. It is hoped that analysis of bone turnover markers can indicate the beginning of bone diseases such as osteoarthritis and osteoporosis so that treatment might start early when it is most effective.

【 授权许可】

CC BY   
© 2009 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.