Enzymatic Polymerization on DNA Modified Gold Nanowire for Label-Free Detection of Pathogen DNA

International Journal of Molecular Sciences

Jaepil Jeong²  Hyejin Kim²  Jong Bum Lee¹ 

[1] Department of Chemical Engineering, University of Seoul, Seoul 130-743, Korea;

关键词: biosensor;    label-free;    pathogen DNA detection;    rolling circle amplification (RCA);    gold nanowire;   

DOI  :  10.3390/ijms160613653

来源: mdpi

【 摘 要 】

This paper presents a label-free biosensor for the detection of single-stranded pathogen DNA through the target-enhanced gelation between gold nanowires (AuNW) and the primer DNAs branched on AuNW. The target DNA enables circularization of the linear DNA template, and the primer DNA is elongated continuously via rolling circle amplification. As a result, in the presence of the target DNA, a macroscopic hydrogel was fabricated by the entanglement of the elongated DNA with AuNWs as a scaffold fiber for effective gelation. In contrast, very small separate particles were generated in the absence of the target DNA. This label-free biosensor might be a promising tool for the detection of pathogen DNAs without any devices for further analysis. Moreover, the biosensor based on the weaving of AuNW and DNAs suggests a novel direction for the applications of AuNWs in biological engineering.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.